Python file hashlib

import hashlib
BLOCKSIZE = 65536
hasher = hashlib.md5()
with open('anotherfile.txt', 'rb') as afile:
    buf = afile.read(BLOCKSIZE)
    while len(buf) > 0:
        hasher.update(buf)
        buf = afile.read(BLOCKSIZE)
print(hasher.hexdigest())

Source: pythoncentral.io

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